A Review of PCR Fundamentals

Course Code: REL-ACU-0-RPCRF

Hours: 1

Type: Online Course

Content Expiration Date: 12/31/2027

Learning Objectives:
Identify the basic components of the polymerase chain reaction (PCR) and their functions within the reaction.
Differentiate between standard PCR, multiplex PCR, and quantitative real-time PCR (qPCR) in terms of their purposes, components, and processes.
Describe four applications of PCR in clinical laboratory testing.

Outline:
Section 1: Polymerase Chain Reaction Chemistry and Experimental Design

Introduction

Reaction Basics

Thermal Cycling Steps

Review

Key Takeaways

Section 2: PCR Application

Types of PCR

Results and Interpretation

Necessary Equipment

Reaction Troubleshooting

PCR Uses in the Diagnostic Laboratory

Review

Key Takeaways

Section 3: Clinical Vignette

Laboratory PCR Testing

Section 4: Conclusion

Course Summary

Course Contributors

References

 

Subject Matter Expert: Leonard Levin, MS, MBA, RM(NRCM), M(ASCP)

Mr. Levin obtained his BS degree in Biochemistry from Albright College, MS degree in Microbiology & Immunology from Temple University, and MBA in Finance from LaSalle University. He worked in nine hospital clinical laboratories for 38 years, including seven years as a microbiology supervisor and 28 years as a laboratory administrative director. Additionally, he worked as an assistant editor for a biology reference publication and editor-in-chief of an AIDS newsletter. He is currently a freelance scientific writer and laboratory management consultant.

Writer: Sooa Devereaux, MSN, RN

Sooa Devereaux, MSN, RN, has a long history of professional development experience within the hospital setting. She has taught in pre-licensure nursing programs and has mentored many nurses, both new graduate and experienced, throughout her career as a nurse educator. Sooa is a Lead SME Writer at Relias and holds an MSN in Nursing Education.

Target Audience:
The target audience for this course is: Clinical Laboratory Personnel; in the following settings: Acute Care Facility.

Relias Learning will be transparent in disclosing if any commercial support, sponsorship or co-providership is present prior to the learner completing the course.

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Course Delivery Method and Format
Asynchronous Distance Learning with interactivity which includes quizzes with questions/answers, and posttests.